Breeder and Distributor of World’s Smallest-Class Laboratory Minipig.


  • Fukuoka University
  • Gifu University
  • Jichi Medical University
  • Keio University
  • Kitasato University
  • Kyoto University
  • Kyushu University
  • Nagoya University
  • The Jikei University School of Medicine
  • Tohoku University

  • Hamri Co., Ltd.
  • IVTeC Co., Ltd.
  • Japan SLC, Inc.
  • Nissei Bilis Co., Ltd.

and more

Collaborating Organizations

  • Marubeni Nisshin Feed Co., Ltd.
  • Joint Research with Swine & Poultry Research Center,
    Shizuoka Prefectural Research Institute of Animal Industry

and more

Related Publications

Among all the publications related to our microminipigs, only those approved by the authors are listed on this website.


  • “Development of a novel porcine ischemia/reperfusion model inducing different ischemia times in bilateral kidneys-effects of hydrogen gas inhalation.”
    Kinoshita Y, Shirakawa K, Sano M, Yokoo T, Kume H, Kobayashi E.
    [Transl Androl Urol. 2022 Apr;11(4):430-438. DOI:10.21037/tau-21-1164]
  • “H2 Inhibits the Formation of Neutrophil Extracellular Traps.”
    Shirakawa K, Kobayashi E, Ichihara G, Kitakata H, Katsumata Y, Sugai K, Hakamata Y, Sano M.
    [JACC Basic Transl Sci. 2022 Jan 12;7(2):146-161. DOI: 10.1016/j.jacbts.2021.11.005.eCollection 2022 Feb.]
  • “A Novel Method for Urinary Tract Reconstruction in Transplanted Embryonic Kidneys Using the Hybrid Stent: A Pig Study.”
    Matsui K, Iwai S, Sasaki H, Takamura T, Kinoshita Y, Saito Y, Fujimoto T, Nakayama Y, Suzuki Y, Matsunari H, Nagashima H, Kobayashi E, Yokoo T.
    [Transplant Direct. 2022 Feb 10;8(3):e1293. DOI:10.1097/TXD.0000000000001293.eCollection 2022 Mar.]


  • “Mammalian enteral ventilation ameliorates respiratory failure.”
    Okabe R, Chen-Yoshikawa TF, Yoneyama Y, Yokoyama Y, Tanaka S, Yoshizawa A, Thompson WL, Kannan G, Kobayashi E, Date H, Takebe T.
    [Med (N Y). 2021 Jun 11;2(6):773-783.e5. DOI:10.1016/j.medj.2021.04.004.Epub 2021 May 14.]
  • “Prevention of Chronic Rejection of Marginal Kidney Graft by Using a Hydrogen Gas-Containing Preservation Solution and Adequate Immunosuppression in a Miniature Pig Model.”
    Nishi K, Iwai S, Tajima K, Okano S, Sano M, Kobayashi E.
    [Front Immunol. 2021 Feb 17;11:626295. DOI:10.3389/fimmu.2020.626295.eCollection 2020.]
  • “Testing of human equivalent dose of health food 5-aminolevulinic acid using the experimental pig.”
    Kobayashi E.
    [Biomed Pharmacother. 2021 Jul;139:111629. DOI:10.1016/j.biopha.2021.111629.Epub 2021 Apr 24.]


  • “Organ Fabrication Using Pigs as An in Vivo Bioreactor.”
    Kobayashi E, Tohyama S, Fukuda K.
    [Keio J Med. 2020 Jun 25;69(2):30-36. DOI: 10.2302/kjm.2019-0006-OA . Epub 2019 Aug 6.]
  • “Low-Flow Nasal Cannula Hydrogen Therapy.”
    Sano M, Shirakawa K, Katsumata Y, Ichihara G, Kobayashi E.
    [J Clin Med Res. 2020 Oct;12(10):674-680. DOI: 10.14740/jocmr4323 . Epub 2020 Sep 21.]
  • “Development of an ex vivo xenogeneic bone environment producing human platelet-like cells.”
    Fujiyama S, Hori N, Sato T, Enosawa S, Murata M, Kobayashi E.
    [PLoS One. 2020 Apr 7;15(4):e0230507.DOI: 10.1371/journal.pone.0230507 . eCollection 2020.]
  • “Viral infections of pigs used for medical education. A Japanese experience.”
    Kobayashi E.
    [Acta Cir Bras. 2020 Sep 21;35(8):e202000808.DOI:10.1590/s0102-865020200080000008.eCollection 2020.]
  • “Preparation and characterization of monoclonal antibodies recognizing two CD4 isotypes of Microminipigs”
    Ohshima, S., Matsubara, T., Miyamoto, A., Shigenari, A., Imaeda, N., Takasu, M., Tanaka, M., Shiina, T., Suzuki, S., Hirayama, N., Kitagawa, H., Kulski, J. K., Ando, A, and Kametani Y.
    [PLoS ONE 2020. DOI:10.1371/journal.pone.0242572] 
  • “Dynamic contrast-enhanced computed tomography lymphangiography with intranodal injection of water-soluble iodine contrast media in microminipig: Imaging protocol and feasibility”
    Tanahashi, Y., Iwasaki, R., Shoda, S., Kawada, H., Ando, T., Takasu, M., Hyodo, F., Goshima, S., Mori, T., Matsuo, M.
    [Eur. Radiol. Eur Radiol. 30: 5913-5922, 2020 DOI:10.1007/s00330-020-07031-0 ]


  • “Genetic association of swine leukocyte antigen class II haplotypes and reproductive traits in Microminipigs”
    Ando, A., Imaeda, N., Matsubara, T., Takasu, M., Miyamoto, A., Oshima, S., Nishii, N., Kametani, Y., Shiina, T., Kulski, J. K and Kitagawa, H.
    [Cells 8 (8): 783, 294-306, 2019. DOI:10.3390/cells8080783]
  • “A Toxoplasma gondii strain isolated in Okinawa, Japan shows high virulence in Microminipigs”
    Taniguchi, Y., Yanagihara, I., Nakamura, Y., Ichikawa, C., Saito, T., Appiah-Kwartenga, C., Matsuzaki, M., Fukumoto, J., Nagamune, K., Kyan, H., Takasu, M., Kitoh, K. and Takashima, Y.
    [Parasitol. Intl. 72, 101935, 2019 DOI:10.1016/j.parint.2019.101935]
  • “Sexual precocity in male microminipigs evaluated immunohistologically using spermatogonial stem cell markers”
    Almunia, J., Nakamura, K., Murakami, M., Mori, T. and Takasu, M.
    [Theriogenology 130, 120‐124, 2019 DOI:10.1016/j.theriogenology.2019.01.040]
  • “Endoscopic ultrasound-guided fine needle injection of hydrogen peroxide into the pancreas: Feasibility and safety study using a survival porcine model”
    Iwashita, T., Uemura, S., Shimizu, M., Hyodo, F., Tomita, H., Iwasaki, R., Takasu, M., Mori, T., Tanaka, H. and Matsuo, M.
    [Ultrasound Med. Biol. 45: 579-585, 2019 DOI:10.1016/j.ultrasmedbio.2018.10.018]
  • “Measurement of J-Tpeakc along with QT-interval prolongation may increase the assay sensitivity and specificity for predicting the onset of drug-induced torsade de pointes: Experimental evidences based on proarrhythmia model animals”
    Goto A, Hagiwara-Nagasawa M, Kambayashi R, Chiba K, Izumi-Nakaseko H, Naito AT, Kanda Y, Sugiyama A.
    [Cardiovasc Toxicol. in press, 2019 DOI:10.1007/s12012-019-09506-z]
  • “Characterization of microminipig as a laboratory animal for safety pharmacology study by analyzing fluvoxamine-induced cardiovascular and dermatological adverse reactions”
    Tanikawa Y, Hagiwara-Nagasawa M, Kambayashi R, Goto A, Chiba K, Kitta K, Hoshiai K, Izumi-Nakaseko H, Naito AT, Sugiyama A.
    [Cardiovasc Toxicol. in press, 2019 DOI:10.1007/s12012-019-09509-w]


  • “Response to estrus induction with abortion treatment in microminipigs on different days after insemination”
    Takasu, M., Maeda, M., Almunia, J., Nakamura, K., Nishii, N. and Takashima, S.
    [J. Reprod. Dev. 64: 361-364, 2018 DOI:10.1262/jrd.2017-107]
  • “Accuracy of follicle count and ovulation confirmation using magnetic resonance imaging in Microminipigs with normal estrus cycles”
    Takasu, M., Baba, R., Owada, S., Nakamura, K., Almunia, J., Nishii, N. and Kitagawa, H.
    [J. Vet. Med. Sci. 80 (1): 125-127, 2018 DOI:10.1292/jvms.17-0417]
  • “Genetic association of swine leucocyte antigen class II haplotypes and body weights in Microminipigs”
    Matsubara, T., Takasu, M., Imaeda, N., Nishii, N., Nishimura, T., Nishimura, T., Shiina, T., Ando, A. and Kitagawa, H.
    [Asian-Aust. J. Anim. Sci. 31 (2): 163-166, 2018. DOI:10.5713/ajas.17.0337]
  • “Electropharmacological characterization of microminipigs as a laboratory animal using anti-influenza virus drug oseltamivir”
    Lubna NJ, Nakamura Y, Hagiwara-Nagasawa M, Goto A, Chiba K, Kitta K, Izumi-Nakaseko H, Ando K, Naito AT, Akie Y, Sugiyama A.
    [J Toxicol Sci. 2018;43:507-512. DOI:10.2131/jts.43.507]
  • “Use of microminipigs for unveiling unknown mechanisms of azithromycin-induced cardiovascular death”
    Goto A, Hagiwara-Nagasawa M, Izumi-Nakaseko H, Kitta K, Hoshiai K, Chiba K, Ando K, Akie Y, Naito AT, Sugiyama A.
    [J Pharmacol Sci. 2018; in press DOI:10.1016/j.jphs.2018.10.003]
  • “Changes of electrocardiogram and hemodynamics in response to dipyridamole: In vivo comparative analyses using anesthetized beagle dogs and microminipigs”
    Ando K, Takahara A, Nakamura Y, Wada T, Chiba K, Goto A, Lubna NJ, Hagiwara-Nagasawa M, Izumi-Nakaseko H, Hoshiai K, Akie Y, Naito AT, Sugiyama A.
    [J Pharmacol Sci. 2018;136:86-92. DOI:10.1016/j.jphs.2018.01.002]
  • “Knocking the chest as a "bridge to pacemaker" -Reply-”
    Wada T, Hagiwara-Nagasawa M, Yoshihara K, Nakazato Y, Lurie KG, Sugiyama A.
    [Circ J. 2018;82:2446-2447. DOI:10.1253/circj.CJ-18-0765]


  • “Pharmacological characterization of microminipig as a model to assess the drug-induced cardiovascular responses for non-clinical toxicity and/or safety pharmacology studies”
    Yokoyama H, Nakamura Y, Saito H, Nagayama Y, Hoshiai K, Wada T, Izumi-Nakaseko H, Ando K, Akie Y, Sugiyama A.
    [J Toxicol Sci. 2017;42:93-101. DOI:10.2131/jts.42.93]
  • “Characterization of microminipigs as an in vivo experimental model for cardiac safety pharmacology”
    Matsukura S, Nakamura Y, Cao X, Wada T, Izumi-Nakaseko H, Ando K, Yamazaki H, Sugiyama A.
    [J Pharmacol Sci.2017;133:103-109. DOI:10.1016/j.jphs.2017.02.002]
  • “Sensitivity and reliability of halothane-anaesthetized microminipigs to assess risk for drug-induced long QT syndrome”
    Cao X, Wada T, Nakamura Y, Matsukura S, Izumi-Nakaseko H, Ando K, Naito AT, Sugiyama A.
    [Basic Clin Pharmacol Toxicol. 2017;121:465-470. DOI:10.1111/bcpt.12838]
  • “Efficacy of precordial percussion pacing assessed in a cardiac standstill microminipig model” Wada T, Ohara H, Nakamura Y, Cao X, Izumi-Nakaseko H, Ando K, Honda M, Yoshihara K, Nakazato Y, Lurie KG, Sugiyama A.
    [Circulation J. 2017;81:1137-1143. DOI:10.1253/circj.CJ-16-1106]


  • “TSST-1 stimulation of SLA-1*0401 protein and mRNA expression in peripheral blood mononuclear cells of SLA-defined Microminipigs”
    Kametani, Y., Ohshima, S., Miyamoto, A., Shigenari, A., Takasu, M., Imaeda, N., Matsubara, T., Tanaka, M., Shiina, T., Kamiguchi, H., Kitagawa, H., Kulski, J. K., Hirayama, N., Inoko, H. and Ando, A.
    [PLoS ONE 11: (10) e0164995, 2016. DOI:10.1371/journal.pone.0164995]
  • “Identification and characterization of two CD4 alleles in Microminipigs”
    Matsubara, T., Nishii, N., Takashima, S., Takasu, M., Imaeda, N., Aiki-Oshimo, K., Yamazoe, K., Kakisaka, M., Takeshima, S., Aida, Y., Kametani, Y., Kulski, J. K., Ando, A. and Kitagawa, H.
    [BMC Vet. Res. 12: 222 (1-12), 2016. DOI: 10.1186/s12917-016-0856-8]
  • “Magnetic resonance imaging of ovarian activity in Microminipigs showing normal estrous cycles”
    Maeda, M., Takashima, S., Takasu, M., Mori, T., Goto, N., Matsubara, T., Almunia, J., Imaeda, N., Ando, A. and Kitagawa, H.
    [In Vivo 30: 35-40, 2016 PMID: 26709126]
  • “Feasible induction of coronary artery vasospasm occurred during cardiac catheterization in a microminipig.”
    Matsukura S, Nakamura Y, Ohara H, Cao X, Wada T, Izumi-Nakaseko H, Ando K, Akasaka Y, Sugiyama A.
    [J Vet Med Sci. 2016;78:873・76. DOI:10.1292/jvms.15-0426]
  • “Coronary artery vasospasms in a microminipig occurred after placing an ameroid constrictor”
    Cao X, Nakamura Y, Aoki T, Wada T, Izumi-Nakaseko H, Ando K, Sakata R, Sugiyama A.
    [J Vet Med Sci. 2016;78:1213-1216. DOI:10.1292/jvms.16-0005]


  • "Hypothermia-Induced J Waves Observed in Microminipig"
    Ando K, Ohara H, Nakamura Y, Cao X, Izumi-Nakaseko H, Sugiyama A.
    [Advances in Animal Cardiology 48 (1):9-13, 2015 DOI:10.11276/jsvc.48.9
  • “Identification of a CD4 variant in Microminipigs not detectable with available anti-CD4 monoclonal antibodies”
    Matsubara, T., Nishii, N., Takashima, S., Takasu, M., Imaeda, N., Aiki-Oshimo, K., Yamazoe, K., Ando, A. and Kitagawa, H.
    [Vet. Immunol. Immunopathol. 168: 176-183, 2015 DOI:10.1016/j.vetimm.2015.09.008]
  • “Body and major organ sizes of young mature Microminipigs determined by computed tomography”
    Takasu, M., Tsuji, E., Imaeda, N., Kaneko, N., Matsubara, T., Maeda, M., Ito, Y., Shibata, S., Ando, A., Nishii, N., Yamazoe, K. and Kitagawa, H.
    [Lab. Anim. 49: 65-70, 2015 DOI:10.1177/0023677214557169]


  • “Characterization of swine leucocyte antigen alleles and haplotypes on a novel miniature pig line, Microminipig”
    Ando, A., Imaeda, N., Ohshima, S., Miyamoto, A., Kaneko, N., Takasu, M., Shiina, T., Kulski, J. K., Inoko, H. and Kitagawa, H.
    [Anim. Gen. 45: 791-798, 2014 DOI:10.1111/age.12199]


  • “Microminipig, a non-rodent experimental animal optimized for life science research. In vivo proarrhythmia models of drug-induced long QT syndrome: development of chronic atrioventricular block model of microminipig”
    Sugiyama A, Nakamura Y, Akie Y, Saito H, Izumi Y, Yamazaki H, Kaneko N, Itoh K.
    [J Pharmacol Sci. 2011;115:122-126 DOI:10.1254/jphs.10R21FM]
  • “Microminipig, a non-rodent experimental animal optimized for life science research”
    Kaneko N, Itoh K, Sugiyama A, Izumi Y.
    [Preface. J Pharmacol Sci. 2011;115:112-114 DOI:10.1254/jphs.10R16FM]